Synthesized peptide derived from human Actin, Muscle Specific
Specificity
This antibody detects endogenous levels of human Actin, Muscle Specific
Source
Mouse IgG
Concentration
1 mg/ml
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Applications
Applications
Species
Dilution
IHC、IF
Human
1: 200-1: 500
Background
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
Immunohistochemical analysis of paraffin-embedded Cardiac muscle. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval.
Immunohistochemical analysis of paraffin-embedded Cardiac muscle. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval.
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .